{"id":1083,"date":"2020-07-09T20:44:58","date_gmt":"2020-07-09T18:44:58","guid":{"rendered":"https:\/\/covid.si\/?p=1083"},"modified":"2020-11-02T21:23:08","modified_gmt":"2020-11-02T20:23:08","slug":"misija-3-iskanje-molekul-ki-se-vezejo-na-rbd-domeno-glikoproteina-s-virusa-sars-cov-2","status":"publish","type":"post","link":"https:\/\/covid.si\/en\/misija-3-iskanje-molekul-ki-se-vezejo-na-rbd-domeno-glikoproteina-s-virusa-sars-cov-2\/","title":{"rendered":"MI 3: Zavrimo COVID-19 – Iskanje molekul, ki se ve\u017eejo na RBD domeno glikoproteina S virusa SARS-CoV-2"},"content":{"rendered":"\t\t
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MI 3: Zavrimo COVID-19 – Iskanje molekul, ki se ve\u017eejo na RBD domeno glikoproteina S virusa SARS-CoV-2<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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STATUS MISIJE:\u00a0REZULTATI V OBDELAVI<\/strong><\/h5>
\u00a0<\/strong><\/div>
NA\u010cRT MISIJE: Said Byadi (Maroko)\u00a0in dr. \u010crtomir Podlipnik<\/strong><\/h5>
\u0160tevilo tar\u010d: 9<\/b><\/h5>
Oznake tar\u010d:\u00a026-34<\/strong><\/h5>
\u0160tevilo spojin: preko\u00a010 M<\/strong><\/h5>
Skupaj sidrano 90 M<\/b>\u00a0spojin<\/h5>
Trajanje misije: 18.05.2020 –<\/strong><\/h5>
Rezultati: v obdelavi<\/strong><\/h5>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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Seznam tar\u010d:<\/strong><\/p>

#26 <\/strong>2ajf<\/strong><\/a> \u2013 <\/strong>Structure of SARS coronavirus spike receptor-binding domain complexed with its receptor.<\/p>

#27 <\/strong>2dd8<\/strong><\/a> – <\/strong>Crystal Structure of SARS-CoV Spike Receptor-Binding Domain Complexed with Neutralizing Antibody<\/p>

#28 <\/strong>3scl<\/strong><\/a> – <\/strong>Crystal structure of spike protein receptor-binding domain from SARS coronavirus epidemic strain complexed with human-civet chimeric receptor ACE2<\/p>

#29 <\/strong>5×58<\/strong><\/a> – <\/strong>Prefusion structure of SARS-CoV spike glycoprotein, conformation 1<\/p>

#30 <\/strong>6ack<\/strong><\/a> –<\/strong>Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, ACE2-bound conformation 3<\/p>

#31 <\/strong>6lzg<\/strong><\/a> – <\/strong>Structure of novel coronavirus spike receptor-binding domain complexed with its receptor ACE2<\/p>

#32 <\/strong>6m17<\/strong><\/a> – <\/strong>The 2019-nCoV RBD\/ACE2-B0AT1 complex<\/p>

#33 <\/strong>6m0j<\/strong><\/a> – <\/strong>Crystal structure of SARS-CoV-2 spike receptor-binding domain bound with ACE2<\/p>

#34 <\/strong>6vw1<\/strong><\/a> – <\/strong>Structure of SARS-CoV-2 chimeric receptor-binding domain complexed with its receptor human ACE2<\/p>

OPIS MISIJE:<\/u> Izbrali smo devet struktur RBD domene proteina glikoproteina S virusa SARS-CoV\/SARS-CoV-2 v kompleksih z ACE2 receptorjem (6vw1, 6m0j, 6m17, 6ack, 3scl, 2ajf)\u00a0 ali protitelesom (2dd8). Za pripravo izhodi\u0161\u010dnih struktur smo najprej uporabili programYASARA\/Structure. RBD domene posameznih proteinskih kompleksov smo prekrili, ter preverili kateri AK ostanki sodelujejo pri interakciji z ACE2, na osnovi teh imformacij smo sedaj omejili obmo\u010dje kamor smo sidrali ligande iz knji\u017enice 10M+ spojin.<\/p>

Za sidranje smo uporabili program RxDOCK, ki smo ga poganjali preko sistema za distribucijsko ra\u010dunanje.<\/p>

Zanimiv rezultat smo dobili s sidranjem konformacijsko omejenih\u00a0 peptidov v vezavno mesto domene RBD proteina. Glej sliko 1:<\/p>

\"\"<\/p>

Slika 1:<\/strong> Vezava konformacijsko omejenega dodekapeptida na RBD domeno glikoproteina Spike virusa SARS-CoV-2.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

STATUS MISIJE: REZULTATI V OBDELAVI   NA\u010cRT MISIJE: Said Byadi (Maroko) in dr. \u010crtomir Podlipnik \u0160tevilo tar\u010d: 9 Oznake tar\u010d: 26-34 \u0160tevilo spojin: […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[10],"tags":[67,59,44,63,68,66,64,37,42,49,43,62,65],"yoast_head":"\nMI 3: Zavrimo COVID-19 - Iskanje molekul, ki se ve\u017eejo na RBD domeno glikoproteina S virusa SARS-CoV-2 - COVID.SI<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/covid.si\/en\/misija-3-iskanje-molekul-ki-se-vezejo-na-rbd-domeno-glikoproteina-s-virusa-sars-cov-2\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"MI 3: Zavrimo COVID-19 - 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